Cell-free DNA Levels Serum Patients with Benign and Malignant Epithelial Ovarian Tumor

Background: An elevated level of cell-free DNA (cfDNA) in the blood circulation has detected in cancer patients in comparison with healthy controls. CfDNA circulation in plasma and serum extensively studied and the results are highly variable due to many factors influence the test results that was preanalytic factors as well as analytic factors. Objectives: Is there any difference in the concentration of serum DNA among patients with benign epithelial ovarian tumors and malignant epithelial ovarian tumors? What is the clinicopathological variable that influences the cfDNA circulation? Method: Venous blood drawn with plain vacutainer, centrifuged at 1,000 rpm for 30 minutes, serum kept in -80 0 C freezer. The cfDNA extracted used NaI method.Results: Collected 30 cases of the benign ovarian tumor and 54 cases of malignant ovarian tumors. The average level serum cfDNA of benign epithelial ovarian tumors and malignant epithelial ovarian tumors were 24.6 ng/mL and 22:29 ng/mL respectively and statistically was not significantly different (p = 0.64). In multivariable analysis with linear regression, there were no clinicopathological variables that statistically significant influence the cfDNA levels in patients with epithelial ovarian tumors where p > 0.05. Conclusion: Concentration of cfDNA circulation of benign epithelial ovarian tumors a little bit higher than malignant epithelial ovarian tumors, but statistically was not significantly different. There was no clinicopathological variable influence the concentration of cfDNA circulation of ovarian tumors.